Tulsa OK, USA) of the microdialysate results were performed on the following samples: the last sample Isoferulic acidCancer
obtained during anaesthesia, the sample obtained during the horse's successful attempt to reach the standing position (sample 0), the samples obtained 1 h and 2 h after standing, and also the sample representing the mean maximum change (increase or decrease) from the end of anaesthesia was seen. The distribution of dialysate glucose was skewed and was log transformed before formal analyses. In all analyses, a p-value of <0.05 was considered significant. Dialysate and plasma results are reported and shown in the figures as means ?standard error of means (SEM). For the statistical analyses, the plasma sample taken at 15 minutes after standing was compared to the dialysate sample collected when the horse regained the standing position (0). In the graphs, these two samples are the point of synchronisation. Since the horses spent different lengths of time lying down in recovery, the samples before time 0 may for different horses represent samples obtained either during anaesthesia or samples obtained after termination of inhalation anaesthesia when still recumbent.Ning up <a href="http://www.ncbi.nlm.nih.gov/pubmed/28110187" title=View Abstract(s)">PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/28110187 to 24 h. Every vial was weighed before and after samplingPage 3 of(page number not for citation purposes)Acta Veterinaria Scandinavica 2009, 51:http://www.actavetscand.com/content/51/1/cose was assayed using modified fluorometric methods . Urea was determined by a spectrophotometric method using PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27007774
standardised reagent kits (Konelab 30, Kone Instruments, Espoo, Finland).Statistical analysis Statistical analyses (Statistica 6.0 and 7.0, StatSoft? Inc. Tulsa OK, USA) of the microdialysate results were performed on the following samples: the last sample obtained during anaesthesia, the sample obtained during the horse's successful attempt to reach the standing position (sample 0), the samples obtained 1 h and 2 h after standing, and also the sample representing the mean maximum change (increase or decrease) from the end of anaesthesia was seen. The timepoint for this sample could be different in individual horses. No statistical analysis was performed on the temporal changes in dialysate during anaesthesia due to the different duration of anaesthesia between horses. Statistical analysis beyond 2 h after standing was not performed.Figure pump 1 An illustration of the microdialysis catheter and infusion An illustration of the microdialysis catheter and infusion pump. The microdialysis catheter consists of a 600mm-long inlet tube, a 90-mm-long double-lumen tube, and a 220-mm-long outlet tube to which the microvial is fastened. The double-lumen tube has a 60-mm-long shaft (0.9 mm in diameter) and a 30-mm tip (0.6 mm in diameter) where the outer layer consists of a polyamide dialysis membrane. The perfusate enters the catheter between the inner tubing and the outer dialysis membrane, allowing for the process of dialysis, the dialysate is subsequently transported away inside the inner tube to be collected in the microvial. The illustration was published with kind permission of CMA/Microdialysis AB, Solna, Sweden.Statistical analyses of blood sample results were performed on the sample obtained before anaesthesia, on the first and last samples taken during anaesthesia, a mean of the samples taken during recovery from anaesthesia when still recumbent, 15 minutes and 1 h and 2 h after regaining the standing position.